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Acute Leukaemia

Acute vs chronic leukaemia:

  • Acute leukaemias (ALL and AML) typically present with rapid onset, and patients are often clinically unwell
  • Chronic leukaemias (CLL and CML) usually have an insidious onset and are frequently diagnosed incidentally

Clinical features within subtypes of acute and chronic leukaemia (i.e. ALL vs AML, and CLL vs CML) are largely overlapping.

Also read the Chronic Leukaemia article.

Acute Lymphoblastic Leukaemia (ALL)

Epidemiology

ALL is the most common childhood cancer [Ref]

  • Bimodal distribution: peak incidence at 5 y/o and 50 y/o (less important)
  • Median age of diagnosis: 14 y/o

For exam purposes (ONLY):

  • Leukaemia in children is almost always ALL
  • Leukaemia in adults is almost never ALL

Aetiology

There is often no specific cause for the development of ALL [Ref]

Key risk factors: [Ref]

  • Inherited genetic syndromes
    • Down syndrome – significantly increases the risk of both AML (500x risk <5 y/o) and ALL (20x risk) [Ref]
    • Li-Fraumeni syndrome
    • Ataxia telangiectasia
  • Certain germline variants are linked to rare familial cases

Clinical Manifestation

Clinical features of ALL can be categorised by the underlying pathophysiology: [Ref]

Underlying pathophysiology Clinical manifestation
Local filtration → bone marrow failure → cytopenia
  • Anaemia → pallor, fatigue, exertional dyspnoea
  • Thrombocytopaenia → petechiae (non-blanching), easy bruisingunexplained bleeding
  • Neutropaenia → persistent / recurrent infection
Extramedullary organ infiltration
  • Hepatosplenomegaly
  • Bone / joint pain → limping child / refusing to bear weight
  • CNS involvement → cranial nerve palsies, leukaemic meningitis
  • Other organ sites include testes, skin, lungs, kidneys
Constitutional symptoms
  • Fever (unexplained)
  • Weight loss (unexplained)
  • Night sweat

Referral Guidelines

The following NICE red flags and referral guidance for leukaemia are particularly important in suspected acute leukaemia, where early recognition and urgent investigation are critical (esp. in children).

Suspected leukaemia in children and young people (<24 y/o) If there is unexplained petechiae OR hepatosplenomegaly → refer for immediate specialist assessment (acute admission / referral within a few hours)
If there are other clinical features of leukaemia → offer a very urgent FBC (within 48 hours)
Suspected leukaemia in adults Consider a very urgent FBC (within 48 hours) in adults with clinical features of leukaemia

Investigation and Diagnosis

As suggested above (in the referral guidelines section), the initial test of choice is a FBC

Laboratory Tests

Test / investigation Interpretation / supportive findings
FBC
  • Anaemia
  • Thrombocytopaenia
  • Variable total white cell count (high / normal / low) – not reliable in distinguishing between ALL and AML
  • Neutropaenia
Peripheral blood smear
  • Numerous lymphoblasts (immature lymphocytes) – large cells with high nuclear-to-cytoplasmic ratio
  • Absence of the following
    • Auer rods (only seen in AML)
    • Smudge cells (only seen in CLL)

Confirmatory Test

Gold standard for diagnosis: bone marrow evaluation (often a bone marrow aspirate)

Morphological assessment >20% of bone marrow lymphoblasts is diagnostic
Immunophenotyping ALL expresses lymphoid antigens:
  • TdT +ve
  • B-cell antigens is more common (CD19, CD22, CD10 etc.)

MPO is -ve in ALL, helping distinguish it from AML (which is MPO +ve, and TdT -ve)

Genetic studies Most significant genetic marker: Philadelphia chromosome BCR-ABL1 oncogene t(9;22)
  • NB the Philadelphia chromosome is characteristic of CML, but can also be seen in ALL
  • Its presence in ALL carries a worse prognosis and affects management

lumbar puncture should be performed in all patients with ALL to check for CNS involvement (for meningeal infiltration and the presence of blasts in the CSF)

Although CNS involvement at diagnosis is relatively uncommon (5-8%), it is important to identify it immediately

  • CNS +ve disease require more intensified treatment
  • Standard systemic chemotherapy does NOT penetrate the BBB / CNS effectively
  • Without testing and prophylaxis, ALL relapses rates in the CNS can reach 75%

Management

Standard management for ALL patients: [Ref]

Systemic chemotherapy Chemotherapy in ALL is made up of a multi-phase protocol, that usually takes 2.5-3 years
  • Induction remission
  • Consolidation
  • Maintenance / delayed intensification (in children)

There is no single standard chemotherapy regimen to learn.

CNS prophylaxis Intrathecal chemotherapy
  • Usually uses lumbar puncture to deliver chemotherapy directly into the CSF
  • Patients generally receive 8-17 doses throughout their treatment

Conditional management: [Ref]

CNS disease at diagnosis These patients require:
  • Standard systemic chemotherapy, and
  • Intensified triple intrathecal chemotherapy
Targeted therapy Tyrosine kinase inhibitors (e.g. ponatinib, imatinib) are used for those with Philadelphia chromosome +ve

Stem cell transplantation (allogeneic primarily) is generally considered in relapsed or high-risk ALL when standard chemotherapy alone is insufficient.

It is used more frequently in adults, as many children with ALL can be cured with chemotherapy alone.

Prognosis

Children have very favourable outcomes [Ref]

  • Cure rates of ~80–90%
  • 5-year overall survival >90%

 

Outcomes in adults are poorer and more variable: [Ref]

  • Survival declines with increasing age (e.g. ~20–30% in patients >60 years)
  • Depends on genetic subtype (e.g. Philadelphia chromosome +ve is associated with worse outcomes)

Acute Myeloid Leukaemia (AML)

Epidemiology

AML is the most common acute leukaemia in adults [Ref]

  • Median age at diagnosis: 68 y/o
  • >2/3 cases are diagnosed in >55 y/o

Aetiology

There is often no specific cause for the development of ALL. [Ref]

Key risk factors: [Ref]

  • Pre-existing haematological disorders (most common)
    • Myelodysplastic syndrome – most significant
    • CML
    • Myeloproliferative neoplasms
  • Previous exposure to cytotoxic agentsradiotherapy
  • Exposure to benzene, petrochemicals (e.g. occupational or environmental exposure)
  • Down syndrome
    • Specifically increases the risk of developing acute megakaryoblastic leukaemia before 5 y/o
    • Significantly increases the risk of both AML (500x risk) and ALL (20x risk) [Ref]

Clinical Manifestation

Clinical features of AML can be categorised by the underlying pathophysiology: [Ref]

Underlying pathophysiology Clinical manifestation
Local filtration → bone marrow failure → cytopenia
  • Anaemia → pallor, fatigue, exertional dyspnoea
  • Thrombocytopaenia → petechiae (non-blanching), easy bruisingunexplained bleeding
  • Neutropaenia → persistent / recurrent infection
Extramedullary organ infiltration
  • Hepatosplenomegaly
  • Gingival hyperplasia
  • Leukaemic cutis – purpuric nodules on the skin
  • CNS involvement (uncommon)
Constitutional symptoms
  • Fever (unexplained)
  • Weight loss (unexplained)
  • Night sweat

AML can also present as oncological emergencies: [Ref]

  • DIC and subsequent intracranial haemorrhage – classically seen in acute pro-myelocytic leukaemia (APL)
  • Significant leukocytosis → leukostasis → respiratory distress, headache/confusion, visual disturbance, stroke-like symptoms

Referral Guidelines

The following NICE red flags and referral guidance for leukaemia are particularly important in suspected acute leukaemia, where early recognition and urgent investigation are critical (esp. in children).

Suspected leukaemia in children and young people (<24 y/o) If there is unexplained petechiae OR hepatosplenomegaly → refer for immediate specialist assessment (acute admission / referral within a few hours)
If there are other clinical features of leukaemia → offer a very urgent FBC (within 48 hours)
Suspected leukaemia in adults Consider a very urgent FBC (within 48 hours) in adults with clinical features of leukaemia

Investigation and Diagnosis

As suggested above (in the referral guidelines section), the initial test of choice is a FBC

Laboratory Tests

[Ref]

Test / investigation Interpretation / supportive findings
FBC
  • Anaemia
  • Thrombocytopaenia
  • Variable total white cell count (high / normal / low) – not reliable in distinguishing between ALL and AML
  • Neutropaenia 

Acute promyelocytic leukaemia (APL) can cause DIC, which present as ↑ PT, ↑ APTT, ↓ fibrinogen, ↑ D-dimer

Peripheral blood smear
  • Numerous myeloblasts (immature myeloid cells) – large cells with high nuclear-to-cytoplasmic ratio
  • Auer rods is classic
  • Absence of smudge cells (only seen in CLL)

Confirmatory Test

Gold standard for diagnosis: bone marrow evaluation (often a bone marrow aspirate) [Ref]

Morphological assessment >20% of bone marrow myeloblasts is diagnostic
Immunophenotyping AML expresses myeloid antigens:
  • MPO +ve
  • CD13 +ve, CD33 +ve

TdT is -ve in AML, helping distinguish it from ALL (which is TdT +ve, and MPO -ve)

Genetic studies Most significant genetic marker: t(15;17) → PML-RARA
  • Seen in acute promyelocytic leukaemia (APL), a distinct subtype of AML
  • It is a haematological emergency due to the high risk of DIC

Management

[Ref]

Acute promyelocytic leukaemia (APL) – confirmed by the presence of PML-RARA Urgent treatment with:
  • All-trans retinoic acid (ATRA), and
  • Arsenic trioxide
Standard AML
  • Induction with the classic 7+3 chemotherapy regimen (7 days of cytarabine and 3 days of anthracycline)
  • Followed by consolidation, which can involve
    • Allogeneic haematopoietic stem-cell transplantation, or
    • Chemotherapy

Allogenic haematopoietic stem cell transplantation is a curative consolidation approach, that is used in:

  • Adverse risk patients (recommended once patient is in remission)
  • High risk of relapse (>35%)
  • Relapse / refractory disease

It is used more commonly in AML than ALL.

Prognosis

Age is one of the most critical prognostic factor in AML [Ref]

  • Survival rates drop significantly as age increases
  • 62% 5-year survival rate in <50 y/o
  • 9.4% 5-year survival rate in >65 y/o

 

Presence of the PML:RARA fusion transcript (hallmark of acute promyelocytic leukaemia) is considered a favourable-risk leukaemia [Ref]

  • >90% cure rate with targeted therapy (all-trans retinoic acid and arsenic trioxide)
  • Despite excellent long-term prognosis, acute promyelocytic leukaemia is notorious for presenting as an oncological emergency

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